Amino acids are the fundamental building blocks of proteins. To learn which amino acids make up a given protein, scientists separate the amino acids using chromatography. Students will perform this same technique using three known amino acids and one unknown protein. By comparing the travel rate and distance of the amino acids, the composition of the protein can be ascertained. This kit contains enough material for up to 15 groups of students. Aligned to Next Generation Science Standards HS-PS1-3 and MS-PS1-2, and explores patterns as a cross cutting concept. In addition to the materials in the kit, a teacher's manual, student study guide copymasters, and material safety data sheets are included.
What's in the box?
- 1 bottle ninhydrin powder (500g)
- 1 bottle 95% ethanol solution (100mL)
- 1 bottle chromatography solvent (375mL)
- 1 bottle alanine control (30mL)
- 1 bottle glycine control( (30mL)
- 1 bottle leucine control (30mL)
- 1 bottle unknown amino acid mixture (30mL)
- 8 capillary tubes
- 20 sheets chromatography paper
WARNING: This product can expose you to chemicals including Methanol/Methyl isobutyl ketone, which is known to the State of California to cause cancer and birth defects or other reproductive harm. For more information go to www.p65warnings.ca.gov.
Safety Data SheetDownload Here
Disciplinary Core Ideas
Cross Cutting Concepts
PLANNING AND CARRYING OUT INVESTIGATIONS
ANALYZING AND INTERPRETTING DATA
Not included in the kit:
Chromatography Chamber (250 mL Beaker or similar)
Parafilm®, plastic wrap, or similar
Kit SpecsComes with instructionsRegulated:
Small Qty Exemption 49CFR173.4Country of Origin:
9023.00.0000CA Prop Warning Statement:
WARNING: This product can expose you to chemicals including Methanol/Methyl isobutyl ketone, which is known to the State of California to cause cancer and reproductive harm. For more information go to www.P65Warnings.ca.gov.