Innovating Science Chromatography of Amino Acids Chemistry Kit (Supplies for 15 Groups)

• Students will perform a chromatography experiment in order to identify the amino acids within an unknown protein
• The chromatography will be run for three known amino acids and one unknown protein
• Hands-on experience with chemical techniques
• Includes enough material for fifteen groups of students
• Kit includes teacher's manual, student study guide copymasters, and material safety data sheets for all included materials

Amino acids are the fundamental building blocks of proteins. To learn which amino acids make up a given protein, scientists separate the amino acids using chromatography. Students will perform this same technique using three known amino acids and one unknown protein. By comparing the travel rate and distance of the amino acids, the composition of the protein can be ascertained. This kit contains enough material for up to 15 groups of students. Aligned to Next Generation Science Standards HS-PS1-3 and MS-PS1-2, and explores patterns as a cross cutting concept. In addition to the materials in the kit, a teacher's manual, student study guide copymasters, and material safety data sheets are included.

What's in the box?

• 1 bottle ninhydrin powder (500g)
• 1 bottle 95% ethanol solution (100mL)
• 1 bottle chromatography solvent (375mL)
• 1 bottle alanine control (30mL)
• 1 bottle glycine control( (30mL)
• 1 bottle leucine control (30mL)
• 1 bottle unknown amino acid mixture (30mL)
• 8 capillary tubes
• 20 sheets chromatography paper

âš  WARNING
WARNING: This product can expose you to chemicals including Methanol/Methyl isobutyl ketone, which is known to the State of California to cause cancer and birth defects or other reproductive harm. For more information go to www.p65warnings.ca.gov.

Safety Data Sheet

Download Here

NGSS

Performance Expectations

HS-PS1-3
MS-PS1-3

Disciplinary Core Ideas

PS1.A
PS1.B

Cross Cutting Concepts

PATTERNS

Engineering Practices

PLANNING AND CARRYING OUT INVESTIGATIONS
ANALYZING AND INTERPRETTING DATA

Items Needed

Not included in the kit:

Paper towels
Chromatography Chamber (250 mL Beaker or similar)
Parafilm®, plastic wrap, or similar

Kit Specs

Comes with instructions

Regulated: Small Qty Exemption 49CFR173.4

Country of Origin: US

Harm Code: 9023.00.0000

CA Prop Warning Statement: WARNING: This product can expose you to chemicals including Methanol/Methyl isobutyl ketone, which is known to the State of California to cause cancer and reproductive harm. For more information go to www.P65Warnings.ca.gov.